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Classical Swine Fever (CSF) Nucleic Acid Test Kit (Lyophilized)

This is a PCR nucleic acid detection reagent kit used to detect Classical swine fever (CSF). Real-time PCR technology has the characteristics of fast, sensitive, highly specific and high accuracy. Utilizing this technology and the FLASHTEST PCR reagent kit, it can accurately diagnose whether pigs carryClassical swine fever (CSF).

Classical Swine Fever (CSF) Nucleic Acid Test Kit (Lyophilized).webp
Classical Swine Fever (CSF) Nucleic Acid Test Kit (Lyophilized).webp
Classical swine fever (CSF), also known as hog cholera, is a highly contagious and often fatal viral disease that affects domestic and wild pigs. It is caused by the classical swine fever virus (CSFV), which belongs to the Pestivirus genus within the Flaviviridae family. CSFV is a single - stranded, positive - sense RNA virus. The disease has a significant impact on the global swine industry, leading to substantial economic losses due to high mortality rates, culling of infected pigs, and restrictions on pig trade.
The CSF nucleic acid detection reagent is designed to specifically identify the presence of CSFV nucleic acid in various swine samples. It mainly utilizes real - time fluorescence quantitative PCR (qPCR) technology. Specific primers and probes are designed based on the conserved regions of the CSFV genome. During the qPCR amplification process, if CSFV nucleic acid is present in the sample, the primers will bind to the target RNA sequence. Reverse transcription is first carried out to convert the RNA into complementary DNA (cDNA), and then PCR amplification occurs. As the DNA polymerase extends the primers, a fluorescent signal is generated and amplified. The real - time fluorescence quantitative PCR instrument can accurately monitor the change of this fluorescent signal. When the signal reaches a pre - set threshold, it can be determined that the sample is positive for CSFV.


Clinical Applications

  1. Early and Accurate Disease Diagnosis:In swine farms, when pigs show symptoms such as high fever, anorexia, hemorrhagic spots on the skin, and neurological symptoms, the CSF nucleic acid detection reagent can be used for rapid diagnosis. By collecting samples like blood, tonsil swabs, or spleen tissues from the sick pigs, veterinarians can quickly determine whether CSFV is the causative agent. Early and accurate diagnosis is crucial as it enables timely implementation of isolation, culling, and disinfection measures to prevent the spread of the virus within the farm. For example, in a large - scale swine farm with sudden onset of illness in some pigs, using this reagent can quickly identify the source of the problem, reducing the economic losses caused by the disease.

  2. Epidemic Surveillance and Prevention:For regions where CSF is endemic or has a high risk of outbreak, regular surveillance using the CSF nucleic acid detection reagent is essential. Local agricultural departments and veterinary institutions can test samples from multiple swine farms in the area at regular intervals. This helps in early detection of the virus, even in asymptomatic carriers. Once the virus is detected, appropriate prevention and control measures can be implemented immediately, such as strengthening biosecurity in pig farms, restricting the movement of pigs, and enhancing vaccination coverage. This can effectively contain the spread of the disease and protect the swine industry in the region.

  3. Quarantine and Import/Export Control:At border checkpoints and quarantine facilities, the CSF nucleic acid detection reagent is used to screen pigs and pork products being imported or exported. This strict screening helps prevent the international spread of CSFV. If a batch of imported live pigs or pork products tests positive for CSFV, appropriate measures can be taken, such as quarantine, destruction of the infected products, or rejection of the shipment, to safeguard the domestic swine industry from the threat of the virus.

  4. Research on CSFV Biology and Vaccine Development:The CSF nucleic acid detection reagent provides valuable support for scientific research on CSFV. It allows researchers to study the genetic characteristics, evolution, and pathogenic mechanisms of the virus. By analyzing a large number of clinical samples, they can gain a better understanding of how CSFV infects pigs, how it mutates, and how the host immune system responds. In the process of vaccine development, this reagent can be used to evaluate the effectiveness of experimental vaccines by detecting the presence of the virus in vaccinated pigs, which is crucial for the development of safe and effective vaccines against CSF.


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Features of Classical Swine Fever (CSF)

This farm animals PCR detection reagent screens for pathogens like Classical swine fever (CSF). It uses drying technology for normal temperature transportation and frozen storage. Other key features include:

  • Disposable pipette, stable pipettor, simplified workflow

  • Internal reference genes are used to verify results, eliminating the need for guessing.

  • Freeze-dried form, transported at room temperature

  • Available in small, flexible packaging that can be ordered according to your needs


Specification of Classical Swine Fever (CSF)

BrandFLASH TEST
Product NameClassical swine fever (CSF) Nucleic Acid Test Kit (Lyophilized)
Product Code/Number3214
Specifications16Test/ Box
Testing ItemsClassical swine fever (CSF)
Testing TargetsSwine
Minimum Detection Limit500 Copies/mL
PrecisionThe coefficient of variation (CV, %) of the Ct values is ≤5%.
Weight0.4kg


Product
Contact With Us
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Blk A4 - F2, Liyuan Development Zone, Binhu District, Wuxi, China

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