Porcine Reproductive And Respiratory Syndrome Virus Type NADC30 (PRRSV-NADC30) Nucleic Acid Test Kit

The PRRSV - NADC30 nucleic acid detection reagent is designed to specifically identify the nucleic acid of this particular strain. It mainly uses real - time fluorescence quantitative PCR technology. Specific primers and probes are designed based on the unique nucleic acid sequences of PRRSV - NADC30. During the PCR amplification process, if the target nucleic acid of PRRSV - NADC30 is present in the sample, the primers will bind to it, and with the extension of DNA strands, a fluorescent signal will be generated and amplified. The real - time fluorescence quantitative PCR instrument can accurately monitor the change of this fluorescent signal, thereby achieving the rapid and accurate detection of PRRSV - NADC30.

Clinical Applications

1. Disease Surveillance in Swine Farms：Regularly test pig samples in swine farms, including blood, nasal swabs, and tissue samples. By using the PRRSV - NADC30 nucleic acid detection reagent, farmers and veterinarians can quickly detect the presence of this virus in the pig herd, especially in the early stage of infection when symptoms may not be obvious. Early detection enables timely implementation of isolation measures for infected pigs, reducing the spread of the virus within the farm. For example, in a large - scale commercial swine farm, sampling and testing can be carried out once a month to ensure the early discovery of potential infections.

2. Epidemic Prevention and Control：In regions where PRRSV - NADC30 has been prevalent or when there is a suspected outbreak, the detection reagent plays a crucial role. It can help in quickly determining the scope of the epidemic by testing samples from multiple sources. Based on the test results, relevant departments can take comprehensive prevention and control measures, such as strengthening biosecurity in pig farms, restricting the movement of pigs, and enhancing disinfection efforts. This helps to effectively contain the spread of the virus and minimize economic losses.

3. Breeding Management：For breeding pig farms, ensuring the health of breeding pigs is essential. Using the PRRSV - NADC30 nucleic acid detection reagent to test breeding pigs before breeding can prevent the introduction of the virus into the breeding population. In addition, during the breeding process, regular testing of sows can help detect potential infections in time, protecting the health of the fetus and improving the survival rate of piglets. This is crucial for maintaining the quality and productivity of the breeding pig herd.

4. Research and Vaccine Development：The detection reagent provides valuable support for scientific research on PRRSV - NADC30. It allows researchers to study the genetic characteristics, transmission patterns, and pathogenic mechanisms of this strain. By analyzing a large number of clinical samples, they can gain a deeper understanding of the virus and develop more effective prevention and control strategies. In the process of vaccine development, the detection reagent can be used to evaluate the effectiveness of experimental vaccines by detecting the presence of the virus in vaccinated pigs, which is of great significance for the development of safe and effective vaccines against PRRSV - NADC30.