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Porcine Diarrhea Panel IV Nucleic Acid Test Kit (Lyophilized)

This is a PCR nucleic acid detection reagent kit used to detect PEDV, TGEV, PDCoV, PRV-A. Real-time PCR technology has the characteristics of fast, sensitive, highly specific and high accuracy. Utilizing this technology and the FLASHTEST PCR reagent kit, it can accurately diagnose whether pigs carryPEDV, TGEV, PDCoV, PRV-A.

Porcine Diarrhea Panel IV Nucleic Acid Test Kit (Lyophilized).webp
Porcine Diarrhea Panel IV Nucleic Acid Test Kit (Lyophilized).webp
Swine viral diarrhea is a major concern in the swine industry, causing significant economic losses due to high morbidity and mortality, especially in piglets. The four - item differential nucleic acid detection reagent targets four key viruses: Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine deltacoronavirus (PDCoV), and Porcine rotavirus A (PRV - A).
PEDV is a highly contagious coronavirus that causes severe diarrhea, vomiting, and high mortality in piglets. TGEV, also a coronavirus, leads to similar symptoms but has different epidemiological and pathogenic characteristics. PDCoV, another member of the coronaviridae family, has emerged in recent years and has become an important pathogen in swine diarrhea cases. PRV - A is a double - stranded RNA virus and is also a common cause of diarrhea in pigs, especially in young animals.
This detection reagent mainly uses multiplex real - time reverse - transcription polymerase chain reaction (RT - qPCR) technology. Specific primers and probes are designed for each of the four viruses based on their unique and conserved nucleic acid sequences. During the RT - qPCR process, the viral RNAs in the sample are reverse - transcribed into cDNAs simultaneously. Then, the cDNAs are amplified in a single reaction tube. Each virus generates a distinct fluorescent signal according to the binding of its specific primers and probes. The real - time fluorescence detection system can precisely monitor and differentiate these signals, enabling rapid and accurate identification of the viruses causing swine viral diarrhea.


Clinical Applications

  1. Accurate Diagnosis in Swine Farms:In swine farms, when pigs show symptoms of diarrhea, it is often difficult to determine which virus is the causative agent. The four - item differential nucleic acid detection reagent can quickly and accurately identify the pathogen. For example, in a farrowing house where many piglets have diarrhea, veterinarians can collect fecal samples or intestinal tissue samples. Using this reagent, they can identify whether it is PEDV, TGEV, PDCoV, or PRV - A within a few hours. This accurate diagnosis is crucial for formulating targeted treatment and prevention strategies. If it is PEDV, strict biosecurity measures and isolation of infected pigs are needed; if it is PRV - A, improving piglet nutrition and immune support may be the focus.

  2. Epidemic Surveillance and Prevention:For regions with a high prevalence of swine viral diarrhea or areas at risk of outbreaks, regular surveillance using this detection reagent is essential. Local agricultural departments and veterinary institutions can test samples from multiple swine farms in the area at regular intervals. By analyzing the detection results, they can understand the prevalence and distribution of these four viruses. Once an increase in the detection rate of a certain virus is found, immediate prevention and control measures can be implemented. For example, if the detection rate of PDCoV increases in a region, strengthening the disinfection of pig farms, restricting the movement of pigs, and enhancing vaccination coverage (if available) can be carried out to prevent the spread of the virus.

  3. Research on Swine Viral Diarrhea Pathogenesis:The four - item differential nucleic acid detection reagent provides a powerful tool for scientific research on swine viral diarrhea. It allows researchers to study the genetic characteristics, transmission patterns, and pathogenic mechanisms of these four viruses. By analyzing a large number of clinical samples from different regions and pig populations, they can gain a deeper understanding of how these viruses infect pigs, how they interact with each other, and how the host immune system responds. This knowledge is crucial for the development of more effective prevention and control strategies, including the development of new vaccines and antiviral drugs.

  4. Vaccine Efficacy Evaluation:In swine farms that have implemented vaccination programs against these viruses, the detection reagent can be used to evaluate the effectiveness of vaccines. After vaccination, testing pigs at regular intervals can determine whether the vaccine has successfully induced an immune response and reduced the viral load. If vaccinated pigs still test positive for a certain virus, it may indicate a vaccine failure or the emergence of a new variant. This information can help in optimizing the vaccination protocol and improving the protection of pigs against swine viral diarrhea.


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Features of Porcine Diarrhea Panel IV

This farm animals PCR detection reagent screens for pathogens like PEDV, TGEV, PDCoV, PRV-A. It uses drying technology for normal temperature transportation and frozen storage. Other key features include:

  • Disposable pipette, stable pipettor, simplified workflow

  • Internal reference genes are used to verify results, eliminating the need for guessing.

  • Freeze-dried form, transported at room temperature

  • Available in small, flexible packaging that can be ordered according to your needs


Specification of Porcine Diarrhea Panel IV

BrandFLASH TEST
Product NamePorcine Diarrhea Panel IV Nucleic Acid Test Kit (Lyophilized)
Product Code/Number3217
Specifications16Test/ Box
Testing Items PEDV, TGEV, PDCoV, PRV-A
Testing TargetsSwine
Minimum Detection Limit500 Copies/mL
PrecisionThe coefficient of variation (CV, %) of the Ct values is ≤5%.
Weight0.4kg


Product
Contact With Us
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+1-707-722-7066
Add:
Blk A4 - F2, Liyuan Development Zone, Binhu District, Wuxi, China

For any inquiries or queries regarding our animal lab test kits and in vitro diagnostic devices, please don't hesitate to reach out to us directly or fill out our online form. We are here to assist you and provide prompt responses to your questions.

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